Description

Other names: 5/6-Carboxyfluorescein succinimidyl ester. 5/6-CFSE.

5/6-CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular lysine residues or other amine containing biomolecules. Due to this covalent binding, fluorescent 5/6-CFSE can be retained within cells for very long periods.

Approximately 7-8 cell divisions can be identified before its fluorescence is too low to be distinguished above the autofluorescence background.

Lymphocyte proliferation, migration and positioning can be simultaneously monitored using 5/6-CFSE.

λex 492 nm; λem 517 nm

Ref.:

1) Fluorescent dyes for lymphocyte migration and proliferation studies.

C.R. Parish, Immunology and Cell Biology. 1999, 77(6): 499–508.

DOI:10.1046/j.1440-1711.1999.00877.x

2) Determination of lymphocyte division by flow cytometry. Journal of Immunological Methods.

A.B. Lyons , C.R. Parish.1994, 171(1), 131–137.

DOI:10.1016/0022-1759(94)90236-4

3) Carboxyfluorescein diacetate succinimidyl ester and the virgin lymphocyte: a marriage made in heaven.

B. Fazekas de St Groth, A.L. Smith, W. P. Koh, L. Girgis, M.C. Cook, P. Bertolino, Immunology and Cell Biology, 1999, 77(6), 530–538.

DOI:10.1046/j.1440-1711.1999.00871.x