Description

Other names: Tetramethylrhodamine-dUTP, Aminohexanoyl-aminoallyl-dUTP-5/6-TAMRA.

This product can be used in enzymatic labeling of DNA by nick (or head) translation tagging technique. It substitutes its natural counterpart dTTP. This way dye-labeled DNAs can be obtained.

Depicted compound has an extended linker between TAMRA and dUTP counterparts, that should allow lower interference during the syntheses of the DNA sequences.

λex 543 nm; λem 575 nm

Ref.:

1) Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

P.W. Rigby, M. Dieckmann, C. Rhodes, Berg P., J. Mol. Biol. 1977, 113 (1), 237–251.

DOI:10.1016/0022-2836(77)90052-3

2) Alignment of the Genomes of Brachypodium distachyon and Temperate Cereals and Grasses Using Bacterial Artificial Chromosome Landing With Fluorescence in Situ Hybridization.

R. Hasterok, A. Marasek, I.S. Donnison, I. Armstead, A. Thomas, I.P. King, E. Wolny, D. Idziak, J. Draper, G. Jenkins, Genetics 2006, 173(1), 349–362.

DOI: 10.1534/genetics.105.049726

3) Insight into the Karyotype Evolution of Brachypodium Species Using Comparative Chromosome Barcoding.

D. Idziak, I. Hazuka, B.  Poliwczak, A. Wiszynska, E. Wolny, R. Hasterok, PLoS One, 2014, 9 (3): e93503.

DOI: 10.1371/journal.pone.0093503