Description

Other names: N-Succinimidyl (acetylthio)acetate, S-Acetylthioglycolic acid NHS ester.

Thiolating reagent for primary amines; thiol can be liberated from protected form under mild conditions.

Can be coupled to molecules containing primary amine by amide bond. The reaction proceeds in buffered solution at pH 7.5.

Deprotection with 0.05M hydroxylamine may be accomplished at neutral pH.

Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates.

Ref.:

1) A new reagent which may be used to introduce sulfhydryl groups into proteins, and its use in the preparation of conjugates for immunoassay.

R.J  Duncan, Anal. Biochem. 1983, 132(1), 68-73.

DOI: 10.1016/0003-2697(83)90426-8