Description

Used in bioanalytical research as a fluorescent label for visualising proteins in living cells. It is excited at 508 nm and emits at 528 nm.

FlAsH-EDT2 selectively binds to proteins containing the tetracysteine (TC) motif CCPGCC and becomes fluorescent when bound. Also can be used to study the processes of protein trafficking in situ.

Ref.:

1) Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins.

S.R. Adams, R.Y. Tsien, Nat. Protoc. 2008, 3(9), 1527–1534.

DOI:10.1038/nprot.2008.144

2)  New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications.

S.R.Adams,  R.E. Campbell, L.A. Gross, B.R. Martin, G.K. Walkup, Y. Yao, J. Llopis, R. Y. Tsien, J. Am. Chem. Soc. 2002, 124(21), 6063–6076.

DOI:10.1021/ja017687n