With two orthogonal reactive groups, this compound is ideal for light-activated ligation to a variety of functionalities by generation of a carbene which will couple irreversibly to groups in close proximity. The alkyne function allows for copper(I)-catalyzed click reactions selectively with azide functionalities.

Ref.:

1) Tandem photoaffinity labeling–bioorthogonal conjugation in medicinal chemistry.

D.J. Lapinsky, Bioorg. Med. Chem., 2012, 20, 6237-6247.

DOI: 10.1016/j.bmc.2012.09.010

2) Reversible Inhibitors Arrest ClpP in a Defined Conformational State that Can Be Revoked by ClpX Association.

A. Pahl, M. Lakemeyer, M. Vielberg, M.W. Hackl, J.Vomacka, V.S. Korotov, M.L.Stein, C.Fetzer, K. Lorenz-Baath, K. Richter, H. Waldmann, M. Groll, S.A. Sieber, Angew. Chem. Int. Ed., 2015, 54, 15892-15896.

DOI: 10.1002/anie.201507266

A water soluble form of SDAD-NHS ester, this crosslinker combines proven amine-reactive NHS and photoreactive diazirine, that is efficiently activated by long-wave UV (330-370 nm). A 13.5Å spacer arm contains a cleavable disulfide thus separating two functionalities upon disulfide reduction. Ideal for extracellular bioconjugations, as this product is cell impermeable.

Ref.:

1) Chemical cross-linking with a diazirine photoactivatable cross-linker investigated by MALDI- and ESI-MS/MS.

A.F. Gomes, F.C. Gozzo, J. Mass. Spectrom. 2010, 45, 892-899.

DOI: 10.1002/jms.1776

An NHS-diazirine based photoreactive crosslinker combines proven amine-reactive NHS and photoreactive diazirine, that is efficiently activated by long-wave UV (330-370 nm). A 13.5Å spacer arm contains a cleavable disulfide thus separating two functionalities upon disulfide reduction. Ideal for intracellular and intramembrane bioconjugations as this product is cell permeable.

Ref.:

1) Chemical cross-linking with a diazirine photoactivatable cross-linker investigated by MALDI- and ESI-MS/MS.

A.F. Gomes, F.C. Gozzo, J. Mass. Spectrom. 2010, 45, 892-899.

DOI: 10.1002/jms.1776

This product contains amine-sensitive NHS group and photo-active diazirine group. It can be used for example in modification of arginine in native peptides for use in chemical probe development.

Ref.:

1) Direct Arginine Modification in Native Peptides and Application to Chemical Probe Development.

V. Grundler, K. Gademann, ACS Med. Chem. Lett. 2014, 5, 1290-1295.

DOI: 10.1021/ml5003508

Can be used for mild conjugation reaction with a peptide or a protein (to SH or NH2). Usual applications for this product: determination of protein 3-D structures, determination of spacial relationships within protein subunits.

Ref.:

1) Positioning of sigma(S), the stationary phase sigma factor, in Escherichia coli RNA polymerase-promoter open complexes.

F. Colland, N. Fujita, D. Kotlarz, J.A. Bown, C.F. Meares, A. Ishihama, A. Kolb, EMBO J. 1999, 18, 4049-4059.

DOI: 10.1093/emboj/18.14.4049

A racemic BABE analog and iron chelating agent. Can be used to couple metal ions to biomolecules.

Ref.:

1) Synthesis of the Protein Cutting Reagent Iron (S)-1-(p-Bromoacetamidobenzyl)ethylenediaminetetraacetate and Conjugation to Cysteine Side Chains.

D. P. Greiner, R. Miyake, J. K. M., A. D. Jones, T. Negishi, A. Ishihama, C. F. Meares, Bioconjugate Chem. 1997, 8, 44-48.

DOI:10.1021/bc9600731