Fluorescent Dyes and Probes – ChemiMart

R27 Dye

Dr. Patrick Durkin — Thu, 27 Nov 2025 10:12:35 +0000

R27 is a light stable benzothiazolium/pyridinium-based dye which finds application in the detection of double stranded DNA. This offers a more stable alternative to SYBR Green I and LC Green Plus, displaying almost no degradation of fluorescence after prolonged exposure (over 6 h) at temperatures up to 95 °C when used in a LightCycler® system for PCR.

λex 440 nm; λem 483-533 nm (dynamic mode)

Cyan 500 NHS Ester

Dr. Patrick Durkin — Thu, 25 Jan 2024 11:17:02 +0000

Cyan 500 NHS Ester is a moderately hydrophilic amine-reactive fluorescent marker. This compound is used to label oligonucleotides, peptides, and other biomolecules bearing an amino-functionality, that can be detected when excited at 450 nm.

λex 450 nm; λem 495 nm

Acridine Orange Hydrochloride Salt, extra pure

Dr. Patrick Durkin — Wed, 12 Jan 2022 09:53:54 +0000

Acirdine Orange is a cell permeable staining dye that intercalates with DNA.

λex 460 nm; λem 650 nm

Coumarin 343 ethylenediamine monoamide

mzaug — Wed, 09 Dec 2020 14:25:32 +0000

Coumarin 343 is a blue emitting fluorophore that can serve as a FRET donor for Fluorescein-based acceptors.

This bears a primary amine functionailty that can be used for amide coupling with a variety of carboxylic acids under standard coupling conditions.

Ref.:

1) A calixarene-based fluorescent ratiometric temperature probe

B. Bardi, I. Tosi, F. Faroldi, L. Baldini, F. Sansone, C. Sissa, F. Terenziani Chemical Communications 2019, 55, 8098-8101

DOI: 10.1039/C9CC04577E

Coumarin 343

mzaug — Wed, 09 Dec 2020 14:23:47 +0000

Coumarin 343 is a blue emitting fluorophore that can serve as a FRET donor for Fluorescein-based acceptors.

Ref.:

1) A julolidine-fused coumarin-NBD dyad for highly selective and sensitive detection of H2S in biological samples

I. Ismail, D. Wang, Z. Wang, D. Wang, C. Zhang, L. Yi, Z. Xi Dyes and Pigments 2019, 163, 700-706.

DOI: 10.1016/j.dyepig.2018.12.064

Fast Blue Dihydrochloride Salt

mzaug — Wed, 09 Dec 2020 14:21:30 +0000

Fast Blue is a diimidine dye used in neuronal research for the non-viral retrograde tracing.

Excitation Wavelength: 365nm
Emission Wavelength: 420nm

4-Trifluoromethyl-7-amino-coumarin, AFC

mzaug — Wed, 09 Dec 2020 14:19:12 +0000

Other names: 4-Trifluoromethyl-7-amino-2H-chromen-2-one. 7-Amino-4-(trifluoromethyl)coumarin. Coumarin 151.

This product can be used in the synthesis of substrates for the fluorometric assay of proteolytic enzymes in biological fluids. May also be used as a calibration standart for AFC-based enzyme substrates.

Compared to AMC, this CF3-containing fluorophore has longer absorption and fluorescence wavelength.

λex 375 nm; λem 480 nm

Ref.:

1) A fluorescent anion sensor that works in neutral aqueous solution for bioanalytical application.

S. Mizukami, T. Nagano, Y. Urano, A. Odani, K. Kikuchi, J. Am. Chem. Soc. 2002, 124, 3920-3925.

DOI:10.1021/ja0175643

7-Aminocoumarin

mzaug — Wed, 09 Dec 2020 14:16:51 +0000

Other names: 2H-1-Benzopyran-2-one, 7-amino-; 7-Amino-2H-1-benzopyran-2-one; 7-amino-2-chromenone; 7-aminochromen-2-one.

This product can be used for studying of proteases through piptide labeling. While 7-amonicoumarin conjugated to a peptide substrate, the fluorescent signal is quenched. After it is cleaved with a protease, AMC is released thus showing fluorescene.

λex 341 nm; λem 440 nm

Br-BDP-APTES

mzaug — Wed, 09 Dec 2020 14:14:38 +0000

Other names: Br-BDP-thiethoxysilane. (3-Aminopropyl)-triethoxysilane modified bromo-tetramethylborondipyrromethenebenzoyl fluorophore.

Can be used for attachment of a fluorescent probe through covalent binding to smart materials or biological objects.

Surfaces containing APTES-functionality have been shown to be nontoxic to embrionic rat cardiomyocytes in vitro.

λex 503 nm; λem 512 nm

Ref.:

1) Growth and Electrophysiological Properties of Rat Embryonic Cardiomyocytes on Hydroxyl- and Carboxyl-Modified Surfaces.

A. Natarajan, C. Chun, J.J. Hickman, P. Molnar, J Biomater Sci Polym Ed. 2008, 19, 1319-1331.

DOI: 10.1163/156856208786052399

Br-BDP-Azide

mzaug — Wed, 09 Dec 2020 14:12:38 +0000

Click-chemistry fluorescent labeling reagent for various sensitive proteins, other biomolecules, or smart materials.

λex 503 nm; λem 512 nm